Part:BBa_I716071:Design

pTrc-(NO SCAR)-rbs lib-beta-rbs lib-alpha

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

Source

Beta portion pcr CA1154F/G00101 on 9145-I716054 (575 bp) Product is ADtemp1 Sub ADtemp1 into pTRC plasmid (Bgl2/XhoI) Product is ADtemp2 pcr CA1099F/D58 on ADtemp2 (3465 bp) Product is ADtemp3 _______________________________________________________ Alpha portion Pcr CA1150F/CA1099R on I716070 (1727 bp) Product is ADtemp5 ____________________________________________________________ Digest ADtemp3 (BsaI/BamHI) Digest ADtemp5 (BsaI/BglII) Ligate, product is: I716071